Gene Summary

Gene:NIT2; nitrilase family member 2
Aliases: HEL-S-8a
Databases:OMIM, HGNC, Ensembl, GeneCard, Gene
Protein:omega-amidase NIT2
Source:NCBIAccessed: 01 September, 2019


What does this gene/protein do?
Show (9)

Cancer Overview

Research Indicators

Publications Per Year (1994-2019)
Graph generated 01 September 2019 using data from PubMed using criteria.

Literature Analysis

Mouse over the terms for more detail; many indicate links which you can click for dedicated pages about the topic.

  • Up-Regulation
  • Cancer Gene Expression Regulation
  • Asparagine
  • Allelic Imbalance
  • 14-3-3 Proteins
  • Signal Transduction
  • Acid Anhydride Hydrolases
  • SFN
  • HeLa Cells
  • Colonic Neoplasms
  • Down-Regulation
  • Messenger RNA
  • Exoribonucleases
  • Subcellular Fractions
  • Caspase 3
  • Lentivirus
  • Nitrogen
  • Exonucleases
  • Aminohydrolases
  • Neoplasm Proteins
  • Cell Cycle Checkpoints
  • FHIT
  • Glutamine
  • Hyperammonemia
  • Proteomics
  • Biomarkers, Tumor
  • Sulfur
  • RNA Interference
  • G2 Phase
  • AKT1
  • omega-amidase
  • Poly(ADP-ribose) Polymerases
  • Amidohydrolases
  • Genetic Vectors
  • nitrilase
  • Colorectal Cancer
  • Chromosome 3
  • Cell Proliferation
Tag cloud generated 01 September, 2019 using data from PubMed, MeSH and CancerIndex

Specific Cancers (1)

Data table showing topics related to specific cancers and associated disorders. Scope includes mutations and abnormal protein expression.

Note: list is not exhaustive. Number of papers are based on searches of PubMed (click on topic title for arbitrary criteria used).

Latest Publications: NIT2 (cancer-related)

Zheng B, Chai R, Yu X
Downregulation of NIT2 inhibits colon cancer cell proliferation and induces cell cycle arrest through the caspase-3 and PARP pathways.
Int J Mol Med. 2015; 35(5):1317-22 [PubMed] Related Publications
Colorectal cancer, also known as colon cancer is the most devastating malignancy worldwide. Previous studies have reported that Nit2, a member of the nitrilase superfamily, is a potential tumor suppressor, although its function remains elusive in colon cancer. In the present study, we employed an RNA interference lentivirus system to silence endogenous NIT2 expression in the colon cancer cell line, HCT116. The knockdown efficiency was determined by RT-qPCR and western blot analysis. The depletion of NIT2 markedly inhibited colon cancer cell proliferation and colony formation and induced cell cycle arrest in the G0/G1 phase, as shown by MTT assay, colony formation assay and flow cytometric analysis, respectively. Further investigation with an intracellular signaling array demonstrated that the depletion of NIT2 triggered the apoptosis of colon cancer cells through the caspase-3 and poly(ADP-ribose) polymerase (PARP) pathways. Our findings suggest that NIT2 may be an oncogene in human colon cancer and may thus serve as a promising therapeutic target for the treatment of colon cancer.

Lin CH, Chung MY, Chen WB, Chien CH
Growth inhibitory effect of the human NIT2 gene and its allelic imbalance in cancers.
FEBS J. 2007; 274(11):2946-56 [PubMed] Related Publications
The mammalian nitrilase (Nit) protein is a member of the nitrilase superfamily whose function remains to be characterized. We now show that the nitrilase family member 2 gene (NIT2) is ubiquitously expressed in multiple tissues and encodes protein mainly distributed in the cytosol. Ectopic expression of Nit2 in HeLa cells was found to inhibit cell growth through G(2) arrest rather than by apoptosis. Consistent with this, proteomic and RT-PCR analyses showed that Nit2 up-regulated the protein and mRNA levels of 14-3-3sigma, an inhibitor of both G(2)/M progression and protein kinase B (Akt)-activated cell growth, and down-regulated 14-3-3beta, a potential oncogenic protein. Genotype analysis in four types of primary tumor tissues showed 12.5-38.5% allelic imbalance surrounding the NIT2 locus. The results demonstrated that NIT2 plays an important role in cell growth inhibition and links to human malignancies, suggesting that Nit2 may be a potential tumor suppressor candidate.

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Cite this page: Cotterill SJ. NIT2, Cancer Genetics Web: Accessed:

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This page in Cancer Genetics Web by Simon Cotterill is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
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